Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.202721 |
Chromosome: | chromosome_9 |
Location: | 3264336 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g387912 | FAP139 | Flagellar Associated Protein, serine endopeptidase | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAAGACCGGGGGCCGAAAGCTCGTAATACT |
Internal bar code: | AGCAGCGAGGGTAGGAAGGGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 649 |
LEAP-Seq percent confirming: | 99.6741 |
LEAP-Seq n confirming: | 14374 |
LEAP-Seq n nonconfirming: | 47 |
LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCTGAGGAGGAGAAGGAGG |
Suggested primer 2: | CAGGGTTACACGATCACACG |