| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202757 |
| Chromosome: | chromosome 14 |
| Location: | 833606 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613200 | Putative metallochaperone; (1 of 5) PTHR13748//PTHR13748:SF4 - COBW-RELATED // COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACCTGCAGCCCCAGGCCGTGCATGGGA |
| Internal bar code: | GTGCAGAGCAGGGTATTGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 99.7732 |
| LEAP-Seq n confirming: | 440 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACCCAGCTGTGTTTAAT |
| Suggested primer 2: | TAGCAGCAGCAGCAGGAGTA |