| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.202811 |
| Chromosome: | chromosome 17 |
| Location: | 5134543 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735200 | KIN1-2,KIN1B | (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin; Kinesin motor protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCATGTCAGGGGTGAGTTGGGCTACACC |
| Internal bar code: | TTTGAATGGCCGAGGCATCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 64.6429 |
| LEAP-Seq n confirming: | 362 |
| LEAP-Seq n nonconfirming: | 198 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGAGGGGTTTGCTTAG |
| Suggested primer 2: | ACTAACAACCCACCCCTTCC |