Insertion junction: LMJ.RY0402.202820_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.202820
Chromosome:chromosome 6
Location:7240601
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g298150 (1 of 230) IPR017986 - WD40-repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATGAAAGGGATGCTCCAGCAACGCATTC
Internal bar code:TGGGTCTAGCGCGCTTGGATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:915
LEAP-Seq percent confirming:99.457
LEAP-Seq n confirming:8242
LEAP-Seq n nonconfirming:45
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTCAGTATCCCACATCGC
Suggested primer 2:GGCCAGTGTGTCCGATTACT