Insertion junction: LMJ.RY0402.202820_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.202820
Chromosome:chromosome 6
Location:7843498
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g302950 (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTCTCGGGCACATTCAGCCCGCCCACCT
Internal bar code:CACACTTGCGCCGAGCCAAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:276
LEAP-Seq percent confirming:99.7563
LEAP-Seq n confirming:2047
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGCGAGTGGAAGTCGG
Suggested primer 2:ACAACAACAACGACCGAACA