| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.202873 |
| Chromosome: | chromosome 14 |
| Location: | 859883 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613400 | POC16 | Proteome of centriole protein 16; (1 of 1) PTHR13720//PTHR13720:SF27 - WD-40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCACCTTCCTGCCGCCCCTCCCGACTT |
| Internal bar code: | CCCACTGCTCAAGGGTCTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 99.4083 |
| LEAP-Seq n confirming: | 168 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCCGTGATGTAGGACAC |
| Suggested primer 2: | GTTATCACCACCACCAACCC |