Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.202881 |
Chromosome: | chromosome 3 |
Location: | 1656178 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g153100 | (1 of 39) IPR016040 - NAD(P)-binding domain | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGTATACTTCTCTAGAGAAATGTCGCC |
Internal bar code: | CAGCGAAGCGGATGTCGTCGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 809 |
LEAP-Seq percent confirming: | 99.7119 |
LEAP-Seq n confirming: | 2769 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCTTCAGGGAGCAAGCTGT |
Suggested primer 2: | TTCGGGACCTGTTTATCGAC |