| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.202885 |
| Chromosome: | chromosome 6 |
| Location: | 2419722 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268450 | (1 of 1) PTHR22850//PTHR22850:SF85 - WD40 REPEAT FAMILY // WD-40 REPEAT-CONTAINING PROTEIN MSI4-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGCGGTGGGTGAGCGGGTGTCAGGAT |
| Internal bar code: | GTAGGAAGAGTTCTATAACATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 99.6863 |
| LEAP-Seq n confirming: | 4131 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCCTGTCAAGCCTCAGT |
| Suggested primer 2: | GAGCAGTGAGTAGCACGCAG |