| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202960 |
| Chromosome: | chromosome 2 |
| Location: | 5295094 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g106650 | CDPK7 | (1 of 6) PTHR24349//PTHR24349:SF23 - SERINE/THREONINE-PROTEIN KINASE // MAP KINASE-ACTIVATED PROTEIN KINASE 5; Calcium/calmodulin-dependent protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAGGAGCATTGGGGATTTAGGTGGTGT |
| Internal bar code: | GTAAGGGCAGAAAACTGTCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1029 |
| LEAP-Seq percent confirming: | 98.5385 |
| LEAP-Seq n confirming: | 4450 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGTCGTTCACACTCTGG |
| Suggested primer 2: | GTCACCACAATGCCAAACAG |