| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202975 |
| Chromosome: | chromosome 15 |
| Location: | 1092332 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638304 | NCL30,OPR74 | Nuclear Control of chloroplast Like 30 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGTTTGGGTATCCGGCCGAAGGGTTGGG |
| Internal bar code: | CGTGTAATTAGCGGTTAACGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 903 |
| LEAP-Seq percent confirming: | 39.0722 |
| LEAP-Seq n confirming: | 1516 |
| LEAP-Seq n nonconfirming: | 2364 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCTGCTCACTGCCTACA |
| Suggested primer 2: | AATGCGGATGTTACAGAGGG |