| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202992 |
| Chromosome: | chromosome 8 |
| Location: | 1952375 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367550 | FAP291 | Flagellar Associated Protein 291 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTAAATTTGAACTGAGCACTGGGCTAAG |
| Internal bar code: | AGGTCGAGGAGAAGGGGCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 895 |
| LEAP-Seq n nonconfirming: | 537 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCACTCCAAGCCCAAGAC |
| Suggested primer 2: | GAGGGTGTGGACTGTGGACT |