| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.202999 |
| Chromosome: | chromosome 1 |
| Location: | 6818309 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049132 | (1 of 1) PTHR23097//PTHR23097:SF105 - TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGCAATAGGCAATAACAAATTACACAC |
| Internal bar code: | AAGCGTTGGGTTATATCGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 767 |
| LEAP-Seq percent confirming: | 99.3426 |
| LEAP-Seq n confirming: | 2418 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGCACACCTTGCATTGA |
| Suggested primer 2: | AGCTCGATACATCTCGGCAT |