Insertion junction: LMJ.RY0402.203019_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.203019
Chromosome:chromosome 2
Location:6241324
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g114150 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGCGCCCGACGGCCCACACGCAATCGAC
Internal bar code:CCGTCTGTGTTGGCTATGAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:300
LEAP-Seq percent confirming:99.6334
LEAP-Seq n confirming:1087
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTCGTGGTATACCGCTTT
Suggested primer 2:CATTCTTTGTGGAGCAGCAA