| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203020 |
| Chromosome: | chromosome 17 |
| Location: | 3110069 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721300 | ASA5 | Mitochondrial F1F0 ATP synthase associated 14.3 kDa protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCATACTCCGTGACAGTGTCCTGACCCC |
| Internal bar code: | GGCTCCGTCTCGTCTTTCCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 99.2165 |
| LEAP-Seq n confirming: | 4812 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACATGCATTTTGGTTGCG |
| Suggested primer 2: | TGAAGTTGCTTCCGGAGTCT |