| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.203020 |
| Chromosome: | chromosome 17 |
| Location: | 3110072 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721300 | ASA5 | Mitochondrial F1F0 ATP synthase associated 14.3 kDa protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGACATAATTTGGGGCTAAATGACGCGG |
| Internal bar code: | TAAGCGTTGCTGGCACCAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1159 |
| LEAP-Seq percent confirming: | 99.7206 |
| LEAP-Seq n confirming: | 4997 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACATGCATTTTGGTTGCG |
| Suggested primer 2: | TGAAGTTGCTTCCGGAGTCT |