| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.203027 |
| Chromosome: | chromosome 1 |
| Location: | 6503088 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g046324 | (1 of 1) PF14906 - Domain of unknown function (DUF4495) (DUF4495) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACTGCGCCGTCCGCGCCGTGCTTCCTCA |
| Internal bar code: | CTCTTCCACCGGGGGTGGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 88 |
| LEAP-Seq percent confirming: | 99.7585 |
| LEAP-Seq n confirming: | 413 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | TATCGCTTGCAACTGTCACC |