| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.203031 |
| Chromosome: | chromosome 9 |
| Location: | 5520787 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400553 | TOR1 | (1 of 1) K07203 - FKBP12-rapamycin complex-associated protein (FRAP); Target of rapamycin 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGCGTTCGGTGGGCGCCGTGCGCAAGTG |
| Internal bar code: | TAGTGATGGAGCGTGCAAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 933 |
| LEAP-Seq percent confirming: | 99.8851 |
| LEAP-Seq n confirming: | 1738 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGGCTGCTGACAGTTCC |
| Suggested primer 2: | GAGAAGTCAACGCTTCGTCC |