Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.203040 |
Chromosome: | chromosome_13 |
Location: | 1782471 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g574900 | FAP228,GTR2 | Glycosyltransferase | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTGAGACCACACATGTCCTCTCCTAGTCC |
Internal bar code: | CCGCGGCTGGCAGCAGAACGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 894 |
LEAP-Seq percent confirming: | 99.6112 |
LEAP-Seq n confirming: | 1281 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTTTCAGGCCCGTCTCTTT |
Suggested primer 2: | CAGATGATGATGTGGAACGC |