| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.203044 |
| Chromosome: | chromosome 3 |
| Location: | 414147 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144927 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACACCACGAGCGCCGGTGAGGCACACC |
| Internal bar code: | TGCCGGAGTGTGTATTGTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 913 |
| LEAP-Seq percent confirming: | 99.0207 |
| LEAP-Seq n confirming: | 6067 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACCAGTCCGGTTTTGTT |
| Suggested primer 2: | TCCAAGTCTCCAACCCTCAC |