| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203048 |
| Chromosome: | chromosome 2 |
| Location: | 5097455 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAACGGCACAACGCCGTCGCCTACCGT |
| Internal bar code: | CTGTGCTATCGACCCGGCCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 978 |
| LEAP-Seq percent confirming: | 94.0387 |
| LEAP-Seq n confirming: | 6373 |
| LEAP-Seq n nonconfirming: | 404 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGACTTGCAAGGGACTTC |
| Suggested primer 2: | AGGTTTGTTTGTGGTCAGGC |