Insertion junction: LMJ.RY0402.203057_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.203057
Chromosome:chromosome 13
Location:3928877
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g590626 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGACAACAGTGCGCAGCTGCCGCTGCCGC
Internal bar code:TTCCCTCGAGAGCTCGGGATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:856
LEAP-Seq percent confirming:97.2044
LEAP-Seq n confirming:2121
LEAP-Seq n nonconfirming:61
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AACCAATTGCAGGTTTGCTC
Suggested primer 2:GTCAGCACTCACGAGTCCAA