| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.203058 |
| Chromosome: | chromosome 16 |
| Location: | 495987 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGTAGAGGAGGAGGCGGAGCTGGTGCT |
| Internal bar code: | AGGGACTTGAGAAGCGCAACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 683 |
| LEAP-Seq percent confirming: | 88.737 |
| LEAP-Seq n confirming: | 9541 |
| LEAP-Seq n nonconfirming: | 1211 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGAATTGTTGGAGCTG |
| Suggested primer 2: | CATGCAAGCGCCTATAAACA |