Insertion junction: LMJ.RY0402.203083_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.203083
Chromosome:chromosome 7
Location:2970512
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g333000 CEP4 (1 of 1) 3.4.22.15 - Cathepsin L; Cysteine endopeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCCCTCGCTGTAGAAGCTGAAGGCCTCG
Internal bar code:GGCACAACACGCACGTTGCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:35
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTTGACACTCACGGCGTC
Suggested primer 2:ATGGCTCTGGAGCAGGACTA