| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203092 |
| Chromosome: | chromosome 7 |
| Location: | 4246594 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341900 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTGCAGGTTGGCGTGCTGGGACCTACC |
| Internal bar code: | CCCAGGCTCGAATCTTGGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 948 |
| LEAP-Seq percent confirming: | 90.4603 |
| LEAP-Seq n confirming: | 5699 |
| LEAP-Seq n nonconfirming: | 601 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTGTGAGTGGTGGGTG |
| Suggested primer 2: | GCGCGTGTGTTAAACAAAGA |