| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.203099 |
| Chromosome: | chromosome_12 |
| Location: | 132311 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g484250 | DHC1,PF9,ROC34,IDA1 | Flagellar inner arm dynein 1 heavy chain alpha (I1/f alpha) | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTTGAGCCGCGCCCACATGGCTGGCAGCT |
| Internal bar code: | TACCGGAGATTCACTTTGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.7161 |
| LEAP-Seq n confirming: | 4566 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCTGGACGAGTACCTGGG |
| Suggested primer 2: | CCTTGCACGTTCTCTCTTCC |