Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.203099 |
Chromosome: | chromosome_12 |
Location: | 132521 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g484250 | DHC1,PF9,ROC34,IDA1 | Flagellar inner arm dynein 1 heavy chain alpha (I1/f alpha) | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTCACCGACCCCTACCAGGTGTCGGAGCG |
Internal bar code: | GTGCGCACTGGCGGTGTGGGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 658 |
LEAP-Seq percent confirming: | 99.6958 |
LEAP-Seq n confirming: | 1311 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACCTGGACGAGTACCTGGG |
Suggested primer 2: | TACCCTGCCCTGTATCCTTG |