Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.203118 |
Chromosome: | chromosome_3 |
Location: | 9115069 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g211521 | FAP165 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AATTTTACGAAGGTCCAGCAGCAGTGTGGA |
Internal bar code: | GTCTAGTGTTGGCGCTGAATGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 818 |
LEAP-Seq percent confirming: | 72.454 |
LEAP-Seq n confirming: | 2675 |
LEAP-Seq n nonconfirming: | 1017 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCACACACCTGCTCGTAGAA |
Suggested primer 2: | CTGTGGGTGGTGAAGAAGGT |