| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.203124 |
| Chromosome: | chromosome 7 |
| Location: | 5517541 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351100 | THB5,PRX | (1 of 1) IPR000104//IPR001486//IPR009050 - Antifreeze protein, type I // Truncated hemoglobin // Globin-like; Truncated hemoglobin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGAAATTCCGCCGCTTGTGATGGGGAT |
| Internal bar code: | ATGTCCTGTGTCGCGAAGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 79.5181 |
| LEAP-Seq n confirming: | 330 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCAACCCCTCTCTCCGT |
| Suggested primer 2: | CCATATTGCCATGTGCTTTG |