| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.203132 |
| Chromosome: | chromosome 14 |
| Location: | 3840349 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632759 | (1 of 2) 4.3.1.19 - Threonine ammonia-lyase / Threonine dehydratase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACTTCGTGAGGGCAGTCGGCGAGGCCG |
| Internal bar code: | AGCGTTTGTTATGTTTTATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1113 |
| LEAP-Seq percent confirming: | 99.3132 |
| LEAP-Seq n confirming: | 3326 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGCTCACGAAAAATAGC |
| Suggested primer 2: | TAGGTAGGAAGCGCATGGAT |