| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.203152 |
| Chromosome: | chromosome 14 |
| Location: | 2514571 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625300 | CAX3 | (1 of 1) PTHR12266//PTHR12266:SF9 - NA+/CA2+ K+ INDEPENDENT EXCHANGER // CATION/CALCIUM EXCHANGER 3-RELATED; CAX family cation antiporter, membrane protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGTGGATCGCCCGAGGTTGGCCTGTGT |
| Internal bar code: | CCTGTCCCTGAGATTTGTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 931 |
| LEAP-Seq percent confirming: | 96.3855 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTCCTAGCCATCCGTTC |
| Suggested primer 2: | GGCATACACGCTCTCTCACA |