| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203158 |
| Chromosome: | chromosome 2 |
| Location: | 3769194 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095400 | BRIX1 | Ribosome biogenesis protein; (1 of 1) K14820 - ribosome biogenesis protein BRX1 (BRX1, BRIX1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGCGGGGTGCCGAGGGCTGAAGGCAGC |
| Internal bar code: | TGTCGCCACGCAGGTCAAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 99.3478 |
| LEAP-Seq n confirming: | 2742 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGATCTTTGCTGGCTCGT |
| Suggested primer 2: | GGCAAGTCCTGGACAACATT |