| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.203167 |
| Chromosome: | chromosome 13 |
| Location: | 3942530 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590750 | HTB21,HTB37 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCTTCAAACCCGGTGTTTATCAACACC |
| Internal bar code: | TTTCGTCTTCTGCTTGCAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 97.0464 |
| LEAP-Seq n confirming: | 230 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGCCTTACCGGTACTTG |
| Suggested primer 2: | GCGTACTTGCCCTTCTTCAG |