| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.203183 |
| Chromosome: | chromosome 7 |
| Location: | 5106967 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347850 | (1 of 1) PF08123 - Histone methylation protein DOT1 (DOT1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCAAGATAGGGTTTTGATTTGTGGCAGC |
| Internal bar code: | ATCGTCGGGCCTTATTTTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1031 |
| LEAP-Seq percent confirming: | 99.2701 |
| LEAP-Seq n confirming: | 952 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTGCATGTATTATCGTG |
| Suggested primer 2: | TGTCTATTGTTGCTGCTGGC |