Insertion junction: LMJ.RY0402.203216_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.203216
Chromosome:chromosome 2
Location:5658697
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g109650 GSP1 Gamete-specific plus 1; (1 of 2) IPR001356//IPR008422//IPR009057 - Homeobox domain // Homeobox KN domain // Homeodomain-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACACCGACCACATACGCTCTCCAGCGC
Internal bar code:TAAATTCGTTTGGACAACGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:723
LEAP-Seq percent confirming:71.0454
LEAP-Seq n confirming:1298
LEAP-Seq n nonconfirming:529
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CACAACACACATTGCCCTTC
Suggested primer 2:GCTGTGCTGTGCTGTATGGT