| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.203245 |
| Chromosome: | chromosome 12 |
| Location: | 1415997 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488000 | FFT4 | putative fructan fructosyltransferase; (1 of 2) 2.4.1.99 - Sucrose:sucrose fructosyltransferase / Sucrose:sucrose 1-fructosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAACATGCATGTGTGCGGCATCACCCTG |
| Internal bar code: | TCCCACCGCGTGGTTCAAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1096 |
| LEAP-Seq percent confirming: | 99.2532 |
| LEAP-Seq n confirming: | 4519 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCAGGGAGAGTGAAGGCA |
| Suggested primer 2: | ATTAACATGTGCGAATGCCA |