Insertion junction: LMJ.RY0402.203280_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.203280
Chromosome:chromosome 10
Location:1979497
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g432400 OPR46 OctotricoPeptide Repeat protein 46 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGGTGCGTGCGCGCTTGACTGAGGCTGG
Internal bar code:GAGGGAATAAGGATGTAGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:891
LEAP-Seq percent confirming:95.3524
LEAP-Seq n confirming:2462
LEAP-Seq n nonconfirming:120
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTTGCTCTGTCGACTCCC
Suggested primer 2:CATCAGAATCTCTGCTGCCA