| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203287 |
| Chromosome: | chromosome 3 |
| Location: | 531678 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145307 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCGCAAGGCGCCAGCATTGCGTCAAA |
| Internal bar code: | TTGAGGCACTGGGTTCGGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 99.8663 |
| LEAP-Seq n confirming: | 747 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCTTTAAGCCCTCCCTT |
| Suggested primer 2: | CTAGCTTTCGACTGAACGGG |