| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203340 |
| Chromosome: | chromosome 3 |
| Location: | 5283739 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183550 | (1 of 3) PTHR15160//PTHR15160:SF4 - VON HIPPEL-LINDAU PROTEIN // PROTEIN F23H11.5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAAAGGTGGCACCAGTAAAAAGCAGACC |
| Internal bar code: | GGTGCACTGCCCTGTGGCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 745 |
| LEAP-Seq percent confirming: | 96.9595 |
| LEAP-Seq n confirming: | 287 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAAGCAAGCGGAAAAGAC |
| Suggested primer 2: | ACGAATGTGGCCGTAGTAGG |