Insertion junction: LMJ.RY0402.203368_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.203368
Chromosome:chromosome 10
Location:974515
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g424775 CTP2,PCH1,PAA1 Copper transporting ATPase and Cu chaperone for CTP1; (1 of 1) PTHR24093:SF124 - COPPER-TRANSPORTING ATPASE PAA1, CHLOROPLASTIC intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATTCACTCCACCCAACATGCCCCTCGTT
Internal bar code:CAATCCGCTCTAAATTCGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:531
LEAP-Seq percent confirming:98.4572
LEAP-Seq n confirming:702
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGGATCGGTCAGAACTTG
Suggested primer 2:GCAGTCGTGTCGCTCAAATA