Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.203368 |
Chromosome: | chromosome 10 |
Location: | 974515 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g424775 | CTP2,PCH1,PAA1 | Copper transporting ATPase and Cu chaperone for CTP1; (1 of 1) PTHR24093:SF124 - COPPER-TRANSPORTING ATPASE PAA1, CHLOROPLASTIC | intron|outside_mRNA |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTCACTCCACCCAACATGCCCCTCGTT |
Internal bar code: | CAATCCGCTCTAAATTCGGTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 531 |
LEAP-Seq percent confirming: | 98.4572 |
LEAP-Seq n confirming: | 702 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCGGATCGGTCAGAACTTG |
Suggested primer 2: | GCAGTCGTGTCGCTCAAATA |