| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.203391 |
| Chromosome: | chromosome 1 |
| Location: | 2558557 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g014850 | (1 of 1) PTHR12281//PTHR12281:SF2 - RP42 RELATED // DCN1-LIKE PROTEIN 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGCGTCTAGGTAAGGACGACGAGGCAC |
| Internal bar code: | CCGACCCTAAAGGTCACCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1057 |
| LEAP-Seq percent confirming: | 99.6103 |
| LEAP-Seq n confirming: | 4345 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACGAATTGTTGCATTGG |
| Suggested primer 2: | CACATGCGGTGAGTGTATCC |