| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203410 |
| Chromosome: | chromosome 16 |
| Location: | 823949 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647602 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATTCTCCCATCCGTTATGGCACACGGA |
| Internal bar code: | TACCGGCACGTGTGAAGCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 88.2647 |
| LEAP-Seq n confirming: | 75875 |
| LEAP-Seq n nonconfirming: | 10088 |
| LEAP-Seq n unique pos: | 113 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGAGTCCTAGCCAAGCC |
| Suggested primer 2: | GACCTCACTGACCTCTTCCG |