| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203449 |
| Chromosome: | chromosome 13 |
| Location: | 3252281 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585900 | LKHA4,LTA4 | (1 of 1) 3.3.2.6 - Leukotriene-A(4) hydrolase / LTA-4 hydrolase; Leukotriene A-4 hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCACCCGCCCAACTGCGTTATTTGCT |
| Internal bar code: | GAAGTCCGGCCCGGGGGAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 42 |
| LEAP-Seq percent confirming: | 99.0453 |
| LEAP-Seq n confirming: | 415 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTATGGGGTTTTGGAGGAT |
| Suggested primer 2: | CCACGACACCTTTTCATCCT |