| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203455 |
| Chromosome: | chromosome 12 |
| Location: | 593033 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492550 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTAGACTGTTCGCTAGGCGAATATCCA |
| Internal bar code: | TATTATCACACGACTGGGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 99.3223 |
| LEAP-Seq n confirming: | 3664 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGTGCTACTGCCCAACC |
| Suggested primer 2: | TCAGACATCGATAGCAACGC |