| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.203464 |
| Chromosome: | chromosome 12 |
| Location: | 8840044 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545000 | ARP7 | (1 of 1) K16615 - actin-related protein 7, plant (PARP7); Actin-related protein, putative SWR-C component | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGACGTTGCCGCTTCTTGCAGCTGTAC |
| Internal bar code: | ACCAACTAGGGCAGCTACATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 99.8044 |
| LEAP-Seq n confirming: | 2551 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGTTTTACCGCATTGCT |
| Suggested primer 2: | GCTGTCGATGGGCCTTATTA |