| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203493 |
| Chromosome: | chromosome 10 |
| Location: | 4215476 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTGGTGAGAGCGGAAGGTTTGGTGTGT |
| Internal bar code: | GCCACATATCGAGTGGAGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.8258 |
| LEAP-Seq n confirming: | 4012 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGCAGACACTGGCAATC |
| Suggested primer 2: | CCGCACACATGGTACTTGTC |