| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.203495 |
| Chromosome: | chromosome 16 |
| Location: | 2661354 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g662000 | HEL62 | (1 of 1) K17679 - ATP-dependent RNA helicase MSS116, mitochondrial [EC:3.6.4.13] (MSS116); DEAD/DEAH box helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGGTGGTTTGGTGATGAGGCTGGGTC |
| Internal bar code: | GGGAGTGCGGTGAGGGGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 283 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTAGGCTTCCATTACTGC |
| Suggested primer 2: | GTGTAACTCCATGGACCGCT |