| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.203497 |
| Chromosome: | chromosome 6 |
| Location: | 3558089 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278116 | (1 of 1) IPR008136 - CinA, C-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATTAATTAAGGAAGTGGTAGACGGTAGG |
| Internal bar code: | AAAGTCGCTCCGTCCTCGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 99.5095 |
| LEAP-Seq n confirming: | 1420 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAGCGGCTGTAGTAGCA |
| Suggested primer 2: | AAACCAACCAAGTCTGGACG |