Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.203536 |
Chromosome: | chromosome 7 |
Location: | 6303599 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g356960 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACACACACACCGGCAGGTCGGTCGG |
Internal bar code: | ACAGGGGCAGTCCGACGGCGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 370 |
LEAP-Seq percent confirming: | 97.4492 |
LEAP-Seq n confirming: | 5807 |
LEAP-Seq n nonconfirming: | 152 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCCACCTCCACACACACAC |
Suggested primer 2: | GTTTGTCATAGCATTCCGGG |