| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.203538 |
| Chromosome: | chromosome 12 |
| Location: | 8850696 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544750 | (1 of 1) PF13637//PF13857 - Ankyrin repeats (many copies) (Ank_4) // Ankyrin repeats (many copies) (Ank_5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGACTGGCTGACTTTTGGACGCTCATCG |
| Internal bar code: | TGGGATGCACTAGACTTCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 322 |
| LEAP-Seq percent confirming: | 98.524 |
| LEAP-Seq n confirming: | 267 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAGGTGAGGCCAGAGAC |
| Suggested primer 2: | GTTTTGTGCAGGCCGTTATT |