| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.203554 |
| Chromosome: | chromosome 3 |
| Location: | 4043109 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172350 | (1 of 1) PF14736 - Protein N-terminal asparagine amidohydrolase (N_Asn_amidohyd) | 3'UTR | |
| Cre03.g172376 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATGGCACGCAGGTGGACCGGAAGGCGC |
| Internal bar code: | TTTGAGCAACCGCCTTCTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 99.724 |
| LEAP-Seq n confirming: | 7587 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCAGTTCTGTGCAAGGGT |
| Suggested primer 2: | TCCCTCCTGTTCCCTTCTTT |